Stopping the Flow Reveals the Reaction Rate
The stop flow mode is based on arresting a selected portion of the sample zone in the detector. If the reaction did not reach equilibrium while the zone was on the way to the detector, a reaction rate curve would be recorded as the reaction product (yellow) is being formed in the detector. Next, when flow resumes, the reacted sample zone is flushed out of the detector, and the baseline is restored. It is difficult to carry out the stop flow technique reproducibly when peristaltic pumps are used to propel carrier and reagent streams. Sequential Injection, syringe-driven systems are more reliable, and their use in stopped flow is very efficient, when performed using lab-on-valve platform (Chapter 2).
1.2.22.
